AB0608 CIRCULATING LEVELS OF TNF/TNFR SUPERFAMILY FACTORS IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS AND ANTIPHOSPHOLIPID RELATED SYNDROMES

Background B-cell tolerance checkpoint defects are part of the pathomechanism in humoral autoimmune disorders, such as Systemic Lupus Erythematosus (SLE) and Antiphospholipid Syndrome (APS) [1]. In APS, despite strong evidence antibody mediated pathogenesis (aPL), specific B cell phenotype abnormalities, development disease patients with inborn errors immunity involving ontogeny, data on use therapies directed toward cells still lacking anticoagulation remains corner stone management [2]. Objectives The aim this study was to measure serum levels TNF/TNFR superfamily factors which involved homeostasis, looking for differences among diseases. Methods Two hundred twenty-seven [75 SLE, 62 SLE+aPL, 36 SLE+APS 54 primary APS (PAPS)] were enrolled. dosage performed by high-sensitivity ELISA. Data expressed median (pg/ml or ng/ml sBCMA) (1 st -3 rd quartile). P values≤0.05 considered statistically significant (*). Results SLE had BAFF levels=316.61 (200.96-579.87) vs 359.26 (SLE+aPL), 615.87* (351.22-893) (SLE+APS), 483.31* (328.02-745.5) (PAPS); APRIL=4563.53 (3028.04-6236.94) 5080.33, 3142.60, 3424.06* (1278.25-5800.38); sBCMA=11891.15 (8859.21-15630.50) 14991.55, 10372.41, 14632.88; sTACI=357.37 (255.05-905.78) 450.51, 424.00, 486.43; sCD40L=1355.97 (651.77-2673.81) 985.42* (498.92-1746.94), 1023.00, 691.96* (258.19-1329.79); TWEAK=2477.49 (1772.76-3069.27) 2454.60, 1683.09* (583-2814.55), 2171.18. a linear regression model including sBCMA, sTACI, BAFF, sCD40L TWEAK predictors, only sBCMA associated positively IgG anticardiolipin (p=0.003) antibeta2-glycoprotein I (p<0.001). second approach built, diagnoses predictor respective TNFF member potential cofounders BMI, gender, CRP, leukocyte levels, hormonal status prednisone (mg/week). Levels clearly underlying diagnosis [predicted values SLE+APS, PAPS: 778.18 (p=0.007), 637.40 (p=0.035), 946.16 (p=0.002), 919.63 (p=0.005); sBCMA: 9068.08 (p=0.004), 10655.50 (p=0.001), 8806.06 (p=0.009), 12131.52 (p=0.001); sCD40L: 2464.84 (p<0.001), 2025.37 2018.68 1943.45 (p=0.002)]. not any other variables included model. APRIL [3017.76 (p=0.016)] SLE+APL [3531.74 (p=0.007)]. Conclusion Our shows an association TNF-family members diagnosis, underscores contribution pathogenesis. On hand, also directly certain anti-phospholipid antibodies positive plasma activation marker antibodies. Taken together, our results reinforcing possible anti-B antiphospholipid related syndromes. Reference [1]Yanaba K, et al. Immunol Rev. 2008; 2. Dieudonné Y, Autoimmun 2021 Acknowledgements: NIL. Disclosure Interests None Declared.